EnzySpin Plasmid Miniprep Kit
Enzyvaa Biotech’s EnzySpin Plasmid Miniprep Kit employs an advanced silica-membrane spin column technology to deliver efficient and reliable plasmid DNA purification. The kit enables recovery of up to 20 μg of high-copy plasmid DNA from a single preparation.
Highlights
- High Efficiency: Yields up to 20 μg of high-quality plasmid DNA
- Rapid Workflow: Complete procedure in less than 14 minutes
- User-Friendly: No phenol/chloroform extraction or alcohol precipitation required
- Ready-to-Use DNA: Purified plasmid DNA is suitable for immediate downstream applications
Specifications
| Application | Plasmid preparation |
| Downstream compatability | PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription |
| Sample Type | Bacteria |
| Yield | 20 ug |
Applications
Ideal for rapid purification of high-purity plasmid DNA compatible with all standard molecular biology techniques, including:
- FastDigest or conventional restriction digestion
- Automated fluorescent or radioactive sequencing
- PCR amplification
- In vitro transcription
- Bacterial transformation
Principle
Bacterial cells are harvested and lysed, followed by clarification of the lysate through centrifugation. The cleared lysate is applied onto a silica spin column, where plasmid DNA selectively binds under high-salt conditions. After sequential washing to remove impurities, the purified DNA is eluted using either the provided elution buffer or nuclease-free water. The resulting plasmid DNA is ready for direct use in downstream workflows such as restriction digestion, PCR, sequencing, transcription, and transformation.
Kit Components
- Resuspension Solution
- Lysis Solution
- Neutralization Solution
- Wash Solution (concentrated)
- RNase A Solution
- Elution Buffer
- Spin Columns
- Collection Tubes
Figures
Video tutorial
Documents
Manuals and Protocols
User guide : EnzySpin Plasmid Isolation Miniprep
Material Safety Datasheets (MSDS)
#CPIM-101