Description

GlowPAGE System is a three component system comprising of GlowPage Gels, a protein visualization dye and GlowTransblot buffer formulation that allows one to resolve proteins in 20-25 min, protein bands development in 2 min and western blot transfer in 5 min using commonly used instrumentation in labs. This system overcomes various limitations of old Laemmli SDS-Page system such as:- 

Protein Modification issue solved: During polymerisation of acrylamide gels, some amount of acrylamide (5-10 mM) remains in unpolymerised form that under alkaline running environment of Laemmli SDS-PAGE, is found to react with Cysteine residues of proteins. Such protein modifications contribute to multiple peaks during Mass Spectrometry analysis. Also, such modifications changes protein migration profile  and sometimes are the reason for multiple bands that we see during western blotting. But, now with the unique functionality of GlowPage Gels, the problem of protein modification is solved to a greater extent. Proprietary modifications, short running time (20-25 min) and a neutral running environment offers these advantanges 

Protein Visualization made easy: Protein staining and destaining for protein bands development used to be a time consuming process. But, now with the functionality of our protein visualisation dye incorporated in GlowPage Gels, one can visualize protein bands immediately after running GlowPage Gels by just UV exposure for 2 min. Most importantly, the same gel can then be used for downstream applications such as western blotting, 2-D and MS. Hence no wastage of resources and time.

Protein loading error issue solved: Variation in sample loading, inconsistent protein transfer efficiency across different lanes of Laemmli Gels are the most common causes for discrepancies seen in lane to lane protein expression profile during western blot data. Although, housekeeping genes were used for data normalization to correct such errors to some extent but they are not full proof. Variation in their expression is reported under different experimental conditions. To solve this problem, whole protein based normalisation is a new norm in the world renowned laboratories. But, now with GlowPage System, a uniformity in protein transfer efficiency and protein imaging on transfer membrane, empower you to perform whole protein based normalization and correct the error in data introduced due to variation in protein loading and inconsistent protein transfer across lanes .