GlowPAGE System Overview

The GlowPAGE System is an integrated, three-component platform comprising GlowPage Gels, a protein visualization dye (GlowProtein Mix), and the GlowTransblot Buffer formulation. Together, these components enable rapid protein separation in SDS-PAGE within 20–25 minutes, protein band visualization in just 2 minutes, and efficient Western blot transfer in 5 minutes, all using commonly available laboratory instrumentation.

This system effectively overcomes several limitations associated with the conventional Laemmli SDS-PAGE system, including :

Protein Modification issue solved: During polymerisation of acrylamide gels, some amount of acrylamide (5-10 mM) remains in unpolymerised form that under alkaline running environment of Laemmli SDS-PAGE, is found to react with Cysteine residues of proteins. Such protein modifications contribute to multiple peaks during Mass Spectrometry analysis. Also, such modifications changes protein migration profile  and sometimes are the reason for multiple bands that we see during western blotting. But, now with the unique functionality of GlowPage Gels, the problem of protein modification is solved to a greater extent. Proprietary modifications, short running time (20-25 min) and a neutral running environment offers these advantanges 

Protein Visualization made easy: Protein staining and destaining for protein bands development used to be a time consuming process. But, now with the functionality of our protein visualisation dye (GlowProtein Mix) and its compatibility with GlowPage Gels, one can visualize protein bands immediately after running GlowPage Gels by just UV exposure for 2 min. Most importantly, the same gel can then be used for downstream applications such as western blotting, 2-D and MS. Hence no wastage of resources and time.

Protein Loading Errors Resolved: Variations in sample loading and inconsistent protein transfer efficiency across lanes in Laemmli gels are among the most common causes of lane-to-lane discrepancies in protein expression profiles observed during Western blotting. Although housekeeping proteins are often used for data normalization to partially correct these errors, they are not foolproof, as their expression levels are known to vary under different experimental conditions. To address these limitations, total (whole) protein–based normalization has emerged as the preferred standard in leading research laboratories worldwide. When GlowProtein Mix is used in combination with GlowPage Gels, it ensures uniform protein transfer efficiency and consistent protein visualization on the transfer membrane. This enables accurate whole protein–based normalization, effectively correcting errors arising from unequal protein loading and inconsistent inter-lane transfer efficiency.

Improved Protein Transfer Efficiency & Reduced Transfer Time: Conventional Laemmli-based transfer buffers often exhibit suboptimal protein transfer efficiency onto widely used nitrocellulose or PVDF membranes. In contrast, our GlowTransblot Buffer significantly enhances transfer efficiency while reducing transfer time to as little as 5 minutes when used with commonly available semi-dry Western blot apparatuses (e.g., Bio-Rad SD Semi-Dry Transfer System).