Description

GlowProtein Mix provides an efficient protein detection system. It does not involve any fixation, staining or destaining. It is directly added in gel mix during the preparation of polyacrylamide gel. After completion of gel run, a short UV light induced photo-activation of protein in the presence of GlowProtein Mix makes the protein fluorescent. These fluorescent protein bands can easily be detected in polyacrylamide gel by UV imager (Gel Doc). Importantly, same polyacrylamide gel can be directly used for protein transfer on Nitrocellulose or PVDF membrane after visualisation of protein bands. Photo-activated protein retains their fluorescent property even after protein transfer on membrane. Hence, the protein bands can also be detected on nitrocellulose or PVDF membrane under UV exposure in Gel Doc system. Protein bands can be visualised multiple times without any additional step or negative effect on downstream applications. The photochemical activation of protein does not affect their immune detection properties, mobility during electrophoresis, and rate of protein transfer. Therefore, GlowProtein Mix is fully compatible with downstream applications such as western blotting, Mass spectroscopy and 2-D electrophoresis. The system is also compatible with SDS or native polyacrylamide gel irrespective of buffer composition and gel percentage.