Description

CloneX vector system is an efficient and straightforward method for cloning PCR products. The procedure exploits inherent terminal transferase activity of Taq Polymerase, which preferentially adds single Adenine (A) at 3’ end of each DNA strand in DNA duplex in the template-independent manner. Hence, most PCR product amplified by Taq DNA polymerase (Taq) possesses single 3’-A overhang. The use of a linearised vector having Thymine (T) overhang at both ends facilitates complementary base pairing of Taq amplified PCR product with cloning vector and hence allows the direct high-efficiency cloning of PCR products.